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See what CRISPR knockdown or knockout of a gene was measured to do, in which cells, and how far each measurement can be trusted.

Biology from anywhere: the measured evidence of published screens, open in your browser or to your AI agent, with no cluster to run and nothing to download.

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TP53 knockdown and knockout, as measured

DDB2 is lower after TP53 knockdown in all 14 screens that measured it and TPX2 is higher in 12 of 14; 6 of the 15 screens that knocked TP53 down have responses consistent within the screen (filled circles under the figure). The knockout screens of TP53 are shown apart from them, never combined.

Tap a cell or a screen name to read it here.

Arrow keys move between cells and the value is read out above the figure. Home and End go to the ends of a row, Enter shows the screens of a folded column. The table view lists every value.

13 of 18 screens drawn.

The gene names and the combined change stay in place while the screens scroll sideways.

Gene
TeloHAEC genome-wide, resting (IGVF), knockdown, reliability 0.72, consistent
TeloHAEC genome-wide, IL-1beta 7 days (IGVF), knockdown, reliability 0.68, consistent
TeloHAEC genome-wide, oxidised LDL 7 days (IGVF), knockdown, reliability 0.63, consistent
TeloHAEC aortic endothelial (GEO GSE210681), knockdown, reliability 0.56, consistent
CD4+ T cells, resting (CZI), knockdown, reliability 0.64, consistent
CD4+ T cells, 48 h stimulated (CZI), knockdown, reliability 0.41, consistent
Jurkat, CD3/CD28 activated (X-Atlas/Pisces), knockdown, reliability 0.23, not shown consistent, its 200 strongest genes consistent
HCT116 (X-Atlas/Pisces), knockdown, reliability 0.20, not shown consistent, its 200 strongest genes consistent
WTC11 cardiomyocytes, TF knockdown (IGVF), knockdown, reliability 0.16, not shown consistent, its 200 strongest genes consistent
K562 genome-wide (Replogle 2022), knockdown, target not read out, not evidence
Melanoma 2686, co-culture with TILs (Frangieh 2021), knockout, reliability 0.01, not shown consistent
Melanoma 2686, control (Frangieh 2021), knockout, target not expressed, not evidence
Melanoma 2686, IFN-gamma (Frangieh 2021), knockout, target not expressed, not evidence
KnockoutCombinedAgree
CombinedAgree
TOP2A
+0.92
+1.10
+0.79
+0.97
+0.06
−0.10
+0.01
+0.04
+0.16
1 of 5 higher
−0.10
+0.14
−0.08
+0.12
+0.141/1 knockout screens agree in direction
+0.499/14 knockdown screens agree in direction
DIAPH3
+0.90
+0.89
+0.69
+0.99
+0.10
0.00
+0.08
+0.08
−0.22
3 of 5 higher
−0.14
+0.13
+0.17
0.00
+0.131/1 knockout screens agree in direction
+0.4611/14 knockdown screens agree in direction
MKI67
+0.80
+1.25
+0.71
+0.79
+0.06
−0.11
+0.06
+0.01
−0.02
2 of 5 higher
−0.11
+0.10
+0.04
+0.10
+0.101/1 knockout screens agree in direction
+0.469/14 knockdown screens agree in direction
TPX2
+0.78
+0.92
+0.67
+0.83
+0.20
+0.08
+0.06
+0.01
+0.20
3 of 5 higher
−0.02
+0.18
−0.05
+0.07
+0.181/1 knockout screens agree in direction
+0.4612/14 knockdown screens agree in direction
ANLN
+0.82
+1.03
+0.76
+0.59
+0.10
+0.03
+0.09
0.00
−0.08
3 of 5 higher
+0.03
+0.13
−0.20
+0.16
+0.131/1 knockout screens agree in direction
+0.4311/14 knockdown screens agree in direction
RRM2
+0.60
+0.92
+0.78
+0.77
+0.26
−0.06
−0.01
+0.07
−0.14
2 of 5 higher
−0.01
+0.11
+0.07
+0.15
+0.111/1 knockout screens agree in direction
+0.428/14 knockdown screens agree in direction
ZMAT3
−0.97
−0.86
−0.67
−0.62
−1.04
−0.54
0.00
−0.26
−0.51
4 of 5 lower
+0.12
+0.03
+0.17
+0.18
+0.030/1 knockout screens agree in direction
−0.6512/14 knockdown screens agree in direction
CDKN1A
−0.73
−0.65
−0.59
−0.78
−0.74
−0.59
0.00
−0.96
−1.02
4 of 5 lower
not measured
+0.18
−0.10
+0.20
+0.180/1 knockout screens agree in direction
−0.6113/14 knockdown screens agree in direction
MDM2
−0.75
−0.79
−0.65
−0.60
−0.75
−0.52
+0.01
−0.35
−0.06
3 of 5 lower
−0.02
+0.02
−0.10
−0.11
+0.020/1 knockout screens agree in direction
−0.5511/14 knockdown screens agree in direction
PTCHD4
−1.09
−0.99
−0.72
−0.85
0.00
0.00
+0.01
−0.29
−0.19
4 of 5 lower
not measured
0.00
0.00
+0.03
0.000/1 knockout screens agree in direction
−0.5010/14 knockdown screens agree in direction
TRIM22
−0.37
−0.57
−0.50
−0.49
−1.06
−0.79
+0.04
0.00
−0.01
4 of 5 lower
not measured
0.00
+0.03
+0.08
0.000/1 knockout screens agree in direction
−0.4911/14 knockdown screens agree in direction
DDB2
−0.59
−0.47
−0.35
−0.42
−0.71
−0.33
−0.13
−0.46
−0.32
5 of 5 lower
not measured
+0.03
−0.07
−0.03
+0.030/1 knockout screens agree in direction
−0.4314/14 knockdown screens agree in direction
−1.0+1.0Change in log expression (natural log): knockdown, against the screen's controls; knockout, against the screen's typical knockout; full colour at −1.0 and +1.0 or beyondnot measured consistent not shown consistentFaded: neither the whole response nor its 200 strongest genes shown consistent within the screen; faded grey: not evidenceFolded column: the share of its screens that moved the gene up and down, not a value. Folding changes only what is drawn: Combined and Agree count every screen.
The response figure of the TP53 page, as measured: the 6 genes that rose and the 6 genes that fell most consistently (rows) in the 15 knockdown screens and 3 knockout screens of TP53 (columns). A copy of the answer of atlas data version 2026-11-12, the version the numbers below count.Studies shown: IGVF Consortium (Engreitz lab) 2026; Zhu et al. 2025 (preprint); Schnitzler et al. 2024; Pan et al. 2026 (preprint); Wang et al. 2026 (preprint); Doctor et al. 2026; IGVF Consortium (Hon lab) 2025; Replogle et al. 2022; Frangieh et al. 2021. Each screen keeps the licence of its source.

The atlas in numbers

Data version 2026-11-12

published screens: 61 CRISPRi knockdown, 21 Cas9 knockout
82
studies
22
cell contexts
41
measured gene-by-context responses
199,063
genes knocked down or out in at least one screen
19,462

What it answers

  • What does knocking down or out my gene do?

    Every screen that knocked it down or out, the genes that moved, and whether each screen's response is consistent within it.

  • What changes my gene?

    The reverse lookup: which knockdowns or knockouts raised or lowered its expression, across the screens that measured it.

  • Does the evidence hold in my cells?

    Whether it comes from your cell line, from other lines, or does not exist, and how often evidence of that kind was confirmed by an independent study.

  • Which knockdowns or knockouts mimic or reverse my signature?experimental

    Paste the genes that go up and down in your experiment; measured knockdowns or knockouts are ranked by how they match or oppose them. A ranking only.